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卡托普利对高压力培养的人脐静脉内皮细胞凋亡的影响

作　者: 赖珩莉
导　师: 罗伟
学　校: 江西医学院
专　业: 内科学
关键词: High pressure condition Middle pressure condition Atmospheric pressure condition Human umbilical veinendothelial cells Proliferatio Apoptosis Captopril
分类号: R540.2
类　型: 硕士论文
年　份: 2000年
下　载: 29次
引　用: 0次
阅　读: 论文下载

内容摘要

1 Objcctivc Tllere is apparent relatio11ship between vascular endothelialcells aPoptosis and llypertension. The pulPose of this paper was to claritywhether different pressure conditions affect the proliferation andapoptosis of cultured human umbi1ical vein endothelial cells, to observethe protect effects of captopl.i1 on HPC-induced HUVECs apoptosis.2 Mcthods Cells were identified as HUVECs by their growtll pattern andmorphologic features. Experiments were carried out using cel1s from the’ 4 th through 6th passages. HUVECs were exposed to either atmosphere,l20, or l80n1mHg pressure by p1acil1g them in a high-tension voltnleterload with 5%co,/air and maintained in RPMI~ l640 supplemented with2.5%ca1f seruln and substrates lbr up to 1 ~ 2 days. \Ve invested thecha1lges of cel1 viability, lnorpho1ogy, agarose gel electrophoresis ofDNA, and flow cytometric a11a1ysis.3 Results The results indicate that HUVECs pro1iferation is influenced bymiddle pressure condition. high pressure condition stimulate proliferationof HUVECs at 16h, however, at 24h and 48h, cel1 11UInber wassigniticant1y lower. Apoptosis cell was markedly increased under highpressul’e conditiOn; "DNA Ladder" was obviously seen at 48h. HoweveT,middle pressure conditiol1 didn’t induce apoptosis. An increase in ce1lviabi1ity and a decrease in apoptosis cel1s were observed in captopril (10-’’ 10-’mol/L) treated HUVECs by a dose-dependence. "DNA Ladder"disappeared after captopril treatment.4 Conclusions (l ) Harvest and hiaintenance of HUVECs was perfOrmed-by modifled Jaffe’s culture method.(2) Ambient pressure in vitro can influence HUVECsgroWth.(3 ) High pressure condition can induce cultured HUVECsapoptosis by a time-dependence.(4 ) Captopril can decrease or inhibit HUVECs apoptosisand reverse groWth inhibition of cultured HUVECsunder high pressure condition.

全文目录

中文摘要  2-3
英文摘要  3-4
英文缩略词表  4-5
前言  5-6
材料与方法  6-10
  一、实验材料  6-7
    （一）细胞来源  6
    （二）主要实验试剂、药品  6
    （三）主要仪器  6-7
  二、实验方法  7-10
    （一）细胞的收集、接种、培养  7
    （二） HUVECs的压力培养  7
    （三） HUVECs增殖的实验分组  7-8
    （四） HUVECs凋亡的实验分组  8
    （五）卡托普利干预作用的实验分组  8
    （六）观察指标及方法  8-10
    （七）统计学处理  10
实验结果  10-16
  一、 HUVECs增殖OD值  10
  二、 HUVECs形态学观察  10-11
  三、 DNA凝胶电泳  11
  四、流式细胞仪  11-16
讨论  16-20
  一、高压力培养对HUVECs增殖的影响及可能机理  16-18
  二、高压力培养对HUVECs凋亡的影响及可能机理  18-19
  三、卡托普列的干预作用及可能机理  19-20
结论  20
致谢  20-21
参考文献  21-25
附图  25-29

卡托普利对高压力培养的人脐静脉内皮细胞凋亡的影响

内容摘要

全文目录

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