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睾酮通过细胞膜雄激素受体影响骨髓巨噬细胞中Notch受体及配体的表达

作　者: 郭东星
导　师: 乔中东
学　校: 四川大学
专　业: 病原生物学
关键词: 巨噬细胞睾酮 Notch 内毒素钙离子浓度膜受体激光共聚焦显微镜
分类号: R339
类　型: 博士论文
年　份: 2004年
下　载: 126次
引　用: 0次
阅　读: 论文下载

内容摘要

1.1.1.研究背景雄激素(androgen)主要指睾酮(testosterone, T)和其在体内的活性代谢产物双氢睾酮(dihydrotestosterone, DHT)。睾酮由睾丸Leydig细胞产生并分泌,其合成和分泌过程受下丘脑-垂体-性腺轴及其它神经体液因素的严密调控。新生成的睾酮进入血液循环后,大部分与性激素结合球蛋白(sex hormone binding globulin, SHBG)结合形成复合物,不能进入组织和细胞;只有约1-2%呈游离状态(血浆浓度约为19.9±4.7nM),能为组织所利用,发挥内分泌调节作用。组织和细胞中的5α-还原酶可将睾酮还原成双氢睾酮,后者与雄激素受体的亲和力要高于睾酮本身。雄激素在生殖系统的作用是:促进胚

全文目录

Part 1 Abstracts  4-18
  1.1 Abstracts in Chinese  4-10
    1.1.1. Research background  4-6
    1.1.2. Resarch purpose  6
    1.1.3. Research protocals  6-7
    1.1.4. Results  7-9
    1.1.5. Conclusion  9
    1.1.6. Key wprds  9-10
  1.2. Abstracts in English  10-18
Part 2 Introduction  18-21
Part 3 Methods and Materials  21-30
  3.1. Mice  21
  3.2. Cell cultures  21-22
  3.3. Purity assay of BMMs  22-23
  3.4. Treatment of BMMs  23
  3.5. Reverse-transcriptase polymerase chain reaction (RT-PCR)  23-25
  3.6. Western blotting assay  25-26
  3.7. Determination of [Ca~(2+)]  26-27
  3.8. Visualization of intracellular androgen receptor  27-28
  3.9. Labeling with testosterone-BSA-FITC  28
  3.10. Confocal laser scanning microscopy assay  28-29
  3.11. Statistical analysis  29-30
Part 4 Results  30-45
  4.1. Assessment of the Purity of BMMs  30-31
  4.2. Expression analysis of Notch in testosterone-treated BMMs  31-35
  4.3. Regulations of testosterone on LPS-induced Notch expression  35-38
  4.4. Testosterone-induced Ca~(2+) mobilization  38-40
  4.5. Absence of androgen receptor  40-41
  4.6. Testosterone binding sites on BMMs  41-45
Part 5 Discussion  45-54
  5.1. Effects of testosterone on BMMs  45-49
  5.2. Mechanism of testosterone action in macrophages  49-54
Part 6 Conclusion  54-56
Part 7 References  56-61
Part 8 Achievements (in Chinese)  61-62
Part 9 Review  62-84
  9.1. Secretion and production of androgen  62-64
  9.2. Biological effects of androgen  64-65
  9.3. Testosterone and immune response  65-67
  9.4. Testosterone and macrophage  67-69
  9.5. Mechanism of testosterone action  69-71
  9.6. Receptors for Rapid Androgen Signaling  71-72
  9.7. Members of Notch signaling pathway  72-74
  9.8 Perspectives  74-84
Part 10 Statement  84-85
Part 11 Acknowledgements  85

睾酮通过细胞膜雄激素受体影响骨髓巨噬细胞中Notch受体及配体的表达

内容摘要

全文目录

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